Quick Overview for CNTF Protein (AA 1-200) (ABIN6387771)
抗原
See all CNTF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-200
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序列
- MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500ng/ml.
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His-IF2DI Tag)CNTF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 2-198) protein (His-IF2DI Tag)CNTF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
- CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 22.9 kDa (200aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000605
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途径
- JAK/STAT Signaling
抗原
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